IVDT_In Vitro Diagnostics Technology

IVD Technology, November/December 2012

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captured by a digital camera. Process- ing and fi ltering techniques defi ne true reacted elements. Multiple custom algorithms query pixel intensity and intensity gradient directionality to determine presence or absence of a signal on each array feature. Once the optical reader software has determined the presence or absence of signal on each array feature, a call logic tree is used to determine the assay result, which is reported automatically. Clinical Indications—BSI Nosocomial infections are an important cause of morbidity and mortality, with 2 million diagnosed cases in the United States each year causing 90,000 deaths.4 1.007 2.007 3.007 4.007 5.007 6.007 7.007 8.007 9.007 10.007 11.007 0.007 species are the leading cause of noso- comial infection worldwide, and up to 60% of all staphylococcal infections are methicillin resistant. Staphylococcus aureus is widely recognized as a signifi - cant pathogen, but the increased use of indwelling devices has implicated Staphylococcal -0.061 0.089 0.239 0.389 0.539 0.689 0.839 0.989 1.139 1.289 1.439 1.589 1.739 1.889 45 50 55 60 65 70 75 Temperature (ºC) Figure 4. Real-time amplifi cation comparison of bpHDA with standard HDA. Panel A: Real-time amplifi cation curves for 100 copies of MRSA genomic DNA. Green lines indicate standard HDA curves. Red lines indicate bpHDA curves. Panel B: Melt peaks for amplifi ed product. Product Tm = 78.5°C. Gold lines indi- cate standard HDA melt profi le. Blue lines indicate bpHDA melt profi le. 80 85 90 95 5 10 15 20 25 Cycles 30 35 40 45 50 ivdtechnology.com IVD TECHNOLOGY | NOVEMBER/DECEMBER 2012 21 -(d/dT) Fluorescence (483-533) Fluorescence (483-533)

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