IVDT_In Vitro Diagnostics Technology

IVD Technology, Spring 2013

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ASSAY DEVELOPMENT the possibility of obtaining better quality or novel antibodies for diagnostic applications. Te simplicity of rabbit IgG gene cloning makes recombinant RabMAb production easy and scalable. Future advances in antibody-based diagnostics will continue to rely on the availability of high-quality or novel antibodies. Signifcant improvements in antibody afnity and specifcity should make diagnosis, prognosis, and companion diagnosis more sensitive, precise, and accurate. Indeed, there are a number of diseases that are under-diagnosed because of a lack of high-quality antibodies. We believe immunodiagnostics with RabMAbs ofers a unique opportunity to address these unmet medical needs. Furthermore, new RabMAbs to targets/epitopes that are not immunogenic or weakly immunogenic in rodents will signifcantly enhance the power of immunoassays and ultimately expand the scope of immunodiagnostics. 28 IVD TEC HNOLO G Y | SP RIN G 2013 magenta cyan yellow black References 1. D Barral and M Brenner, "CD1 antigen presentation: how it works," Nature Reviews / Immunology 7 (2007): 929-941. 2. RG Mage, D Lanning, KL Knight, "B cell and antibody repertoire development in rabbits: Te requirement of gut-associated lymphoid tissues," Development & Comparative Immunology 30 (2006): 137–153. 3. RM Krause, "Te search for antibodies with molecular uniformity," Adv Immunol. 12 (1970): 1-56. 4. RM Krause, "Experimental approaches to homogenous antibody populations: factors controlling the occurrence of antibodies with uniform properties," Fed Proc. 29 (1970): 59-65. 5. JC Bystryn, JS Jacobsen, P Liu et al., "Comparison of cell surface human melanomaassociated antigens identifed by rabbit and murine antibodies," Hybridoma 1 (1982): 465-472. 6. A Weller, J Meek, ED Adamson, "Preparation and properties of monoclonal and polyclonal antibodies to mouse epidermal growth factor (EGF) receptors: evidence for cryptic EGF receptors in embryonal carcinoma cells," Development 100 (1987): 351-363. 7. TJ Raybould, M Takahashi, "Production of stable rabbit-mouse hybridomas that secrete rabbit mAb of defned specifcity," Science 240 (1988): 1788-1790. 8. H Spieker-Polet, P Sethupathi, PC Yam et al., "Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas," Proc Natl Acad Sci USA, 92 (1995): 9348-9352. 9. W Zhu and G-L Yu, "Rabbit Hybridoma," in Terapeutic Monoclonal Antibodies: from bench to clinic, (Wiley, 2009) 151-168 10. Y Yu et al., "A Humanized Anti-VEGF Rabbit Monoclonal Antibody Inhibits Angiogenesis and Blocks Tumor Growth in Xenograft Models," PLoS ONE 5 (2010): 2-12 11. S Rossi et al., "A Comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies," Am J Clin. Pathol. 124 (2005): 295-302. 12. Z Huang et al., "Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fxed, parafn-embedded tissue sections," Appl Immunohistochem Mol Morphol. 13 (2005): 91-95. 13. K Park et al., "Antibody-based detection of ERG rearrangement-positive prostate cancer," Neoplasia 12 (2010): 590-598. 14. J Allen, "A semisynthetic epitope for kinase substrates," Nature Methods 4 (2007): 511516. IVD Weimin Zhu is cofounder and senior vice president, Antibody Technology, at Epitomics, an Abcam Co., in Burlingame, CA. He can be reached at weimin. zhu@epitomics.com. i v d t e c hnol ogy. com ES237188_IV1305_028.pgs 04.25.2013 01:20 UBM

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